consider the equation for the resolution of a separation resolution n4 1 kbkb 1 where n 1

/in /by

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Consider the equation for the resolution of a separation

resolution=√N4(α−1)αkBkB+1

where N

is the number of theoretical plates,
α is the relative retention or selectivity factor, and
kB

is the retention factor of the slower moving compound.

Which of the methods can be used to improve the resolution between two compounds for a liquid separation using a packed chromatography column?

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